منابع مشابه
Inhibition of human pancreatic ribonuclease by the human ribonuclease inhibitor protein.
The ribonuclease inhibitor protein (RI) binds to members of the bovine pancreatic ribonuclease (RNase A) superfamily with an affinity in the femtomolar range. Here, we report on structural and energetic aspects of the interaction between human RI (hRI) and human pancreatic ribonuclease (RNase 1). The structure of the crystalline hRI x RNase 1 complex was determined at a resolution of 1.95 A, re...
متن کاملPurification and immunological characterization of human pancreatic ribonuclease.
Three alkaline RNases were purified from human pancreatic juice to near homogeneity as judged by sodium dodecyl sulfate and native polyacrylamide gel electrophoreses. The molecular weights of these RNases determined by sodium dodecyl sulfate electrophoresis were 27,000, 19,000, and 13,000. The activities of these three RNases were increased by addition of Mg2+, Ca2+, Co2+, K+, Na+, spermine, an...
متن کاملEndowing human pancreatic ribonuclease with toxicity for cancer cells.
Onconase is an amphibian protein that is now in Phase III clinical trials as a cancer chemotherapeutic. Human pancreatic ribonuclease (RNase 1) is homologous to Onconase but is not cytotoxic. Here, ERDD RNase 1, which is the L86E/N88R/G89D/R91D variant of RNase 1, is shown to have conformational stability and ribonucleolytic activity similar to that of the wild-type enzyme but > 10(3)-fold less...
متن کاملRadioimmunoassay for human pancreatic ribonuclease and measurement of serum immunoreactive pancreatic ribonuclease in patients with malignant tumors.
A method for radioimmunoassay of human pancreatic RNase was developed. The method is sensitive, reproducible, and specific. Almost no cross-reactivity exists between human pancreatic and liver RNases. A good correlation was observed between the serum concentration of pancreatic RNase as measured by radioimmunoassay and its enzymatic activity using polycytidylic acid as substrate. The concentrat...
متن کاملReversible denaturation of pancreatic ribonuclease by urea.
Studies of the denaturation of proteins indicate certain characteristics of the forces which normally constrain them in their native chain conformations. In the case of bovine pancreatic ribonuclease, the description of the noncovalent bonds involved has taken on heightened interest following the recent report by White (1) that the integrity of the 4 disulfide bonds does not appear to be prereq...
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ژورنال
عنوان ژورنال: Cancer
سال: 2000
ISSN: 0008-543X,1097-0142
DOI: 10.1002/1097-0142(20000915)89:6<1252::aid-cncr9>3.3.co;2-3